The Nocardial aph(2″) Gene Confers Tobramycin and Gentamicin Resistance and Is an Effective Positive Selection Marker in Mycobacteria and Nocardia

The current study aimed to evaluate the feasibility of using aminoglycoside 2″-O-phosphotransferase aph(2″) gene as a positive selection marker in N. asteroides, M. smegmatis, abscessus and tuberculosis. gene, known confer resistance tobramycin, was PCR amplified from farcinica cloned into two plasmid vectors, pMSG383 pDB151, harboring hygromycin zeocin markers, respectively. recombinant plasmids were transformed target microorganisms, selectability assessed against varying concentrations tobramycin an E-test gentamicin. results indicated that is useful Mycobacteria Nocardia with good at 2.5–10 µg/mL for smegmatis mc2-155 asteroides ATCC 19,247, 60–160 19,977 tuberculosis H37Ra. minimum inhibitory concentration (MIC) gentamicin >256 µg/mL, whereas respective MIC wild-type strains 0.125 0.38 8 These findings demonstrate potential genetic manipulation processes Nocardia, thus facilitating their research improving efficiency biotechnology applications. Conclusions: useful, new various Mycobacteria.